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4 changes: 2 additions & 2 deletions 3DFISHinCelegans/NewFishPureCP.cppipe
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@@ -1,6 +1,6 @@
CellProfiler Pipeline: http://www.cellprofiler.org
Version:5
DateRevision:421
DateRevision:428
GitHash:
ModuleCount:20
HasImagePlaneDetails:False
Expand Down Expand Up @@ -251,7 +251,7 @@ SaveImages:[module_num:19|svn_version:'Unknown'|variable_revision_number:16|show
ExportToSpreadsheet:[module_num:20|svn_version:'Unknown'|variable_revision_number:13|show_window:True|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False]
Select the column delimiter:Comma (",")
Add image metadata columns to your object data file?:No
Add image file and folder names to your object data file?:Yes
Add image file and folder names to your object data file?:No
Select the measurements to export:Yes
Calculate the per-image mean values for object measurements?:No
Calculate the per-image median values for object measurements?:No
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Expand Up @@ -19,8 +19,6 @@ and the clustering of their nuclei in 3D cell culture.

Voxel dimensions: 0.124x0.124x0.200 um

Calibration Image: Voxel dimensions: 1x1x1 um

## **Importing data in CellProfiler**

1. Highlight the **Images** module.
Expand Down Expand Up @@ -56,8 +54,7 @@ always a good practice to check if everything is fine.


>The relative pixel spacing was provided and is 0.124 um in x and y and
0.200 um in z. To run the calibration file please change the relative
pixel spacing to 1x1x1 um.
0.200 um in z.

6. Assign the images “variable names” that describe the contents in the image. For example, use the name "Nuclei", "DNA", “DAPI” or something else that will remind you what the image is.
7. Hit the "update" button to populate
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